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PR.CRY-SAL Ag
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Product Overview
The Pet Reptile Cryptosporidium-Salmonella Ag Combo Test is a lateral flow immunochromatographic assay designed for the rapid differential diagnosis of these pathogens by detecting Cryptosporidium serpentis and Salmonella spp. antigens in reptile cloaca secretions or feces, from a single specimen collection.
Suitable for:
🦎 Pet reptiles at risk of infection 🏥 Veterinary clinics & pet stores 🐾 Reptile keepers & breeders 🔬 Field screening
Key Features
| 🎯 Multi-marker panel | Up to 2 analytes in one test — single specimen, one preparation |
| 🧬 Target | Cryptosporidium serpentis and Salmonella spp. antigens |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 5T kit available |
Detection Principle
The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 2 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.
Test Procedure
| 1 | Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect sample — collect fresh feces with the swab from the anus or from the ground — the amount should cover approximately 1/3 to 2/3 of the swab tip. |
| 3 | Prepare sample — insert the swab into the assay buffer tube, agitate thoroughly, and let it stand for 30–60 seconds. Use the liquid supernatant as sample. |
| 4 | Apply sample — remove the test card from the foil pouch, place it flat, and transfer 4 drops of the treated sample into each sample well. |
| 5 | Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing. |
NOTE: In case of no flow in 30 seconds, add 1 more drop of the sample.
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed in the corresponding window; repeat with a new test card. |
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD101 |
| Target | Cryptosporidium serpentis and Salmonella spp. antigens |
| Specimen | Reptile cloaca secretions or feces |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (5T) | 5 CRY-SAL Ag test cards, 5 vials of assay buffer, 5 disposable droppers, 5 specimen collection swabs, 1 instruction for use |
| Pack size | 5 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Reptile cloaca secretions or feces. |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD101 | Pet Reptile Cryptosporidium-Salmonella Ag Combo Test | 5 tests / box |
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