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CDV Ag-CPV Ag-CHW Ag-EHR Ab-LEPTO Ab
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Product Overview
The Canine Distemper Virus-Parvovirus-Dirofilaria immitis Ag, Ehrlichia canis-Leptospira spp. Ab Combo Test is a lateral flow immunochromatographic assay designed for shelter and rescue health screening by detecting canine distemper virus antigen in secretions from the eye conjunctiva, nasal cavities or anus, canine parvovirus antigen in feces, and Dirofilaria immitis antigen together with antibodies to Ehrlichia canis and Leptospira spp. in serum, plasma or whole blood, from a single test card.
Suitable for:
🐕 Dogs at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & breeding kennels 🔬 Field screening
Key Features
| 🎯 Multi-marker panel | Up to 5 analytes in one test — single specimen, one preparation |
| 🧬 Target | Canine distemper virus, canine parvovirus and Dirofilaria immitis antigens, and Ehrlichia canis and Leptospira spp. antibodies |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 10T kit available |
Detection Principle
The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 5 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.
Test Procedure
| 1 | Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect samples — collect secretions from the eye conjunctiva, nasal cavities or anus with a swab for the CDV window and collect fresh feces with a separate swab (covering about 1/3 to 2/3 of the swab tip) for the CPV window; collect whole blood into a vacuum blood collection tube or obtain serum or plasma by centrifugation for the CHW, EHR and LEPTO windows. |
| 3 | Prepare samples — insert the CDV swab into the CDV assay buffer tube and the CPV swab into the CPV assay buffer tube, agitate each swab in its matching buffer, and use the liquid supernatant as the treated sample for the CDV and CPV windows; serum, plasma or whole blood is used directly without dilution. |
| 4 | Apply samples — remove the test card from the foil pouch and place it flat on a work surface. Transfer 3 drops of the treated CDV sample into the CDV sample well and 4 drops of the treated CPV sample into the CPV sample well; for each of the CHW, EHR and LEPTO sample wells, add 1 drop of serum, plasma or whole blood with a capillary dropper, then 3 drops of the CHW-EHR-LEPTO assay buffer with a disposable dropper. |
| 5 | Read result — start the timer and read the result at 5–10 minutes. Do not move the test card during testing. |
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed in the corresponding window; repeat with a new test card. |
The five windows are read independently, and any faintly visible T line should be considered positive when the C line is also present. The CDV window detects canine distemper virus antigen in secretions from the eye conjunctiva, nasal cavities or anus; the CPV window detects canine parvovirus antigen in feces; the CHW, EHR and LEPTO windows detect Dirofilaria immitis antigen and antibodies to Ehrlichia canis and Leptospira spp., respectively, in serum, plasma or whole blood. Read the result at 5–10 minutes after sample application; a window in which no line appears in the C zone is invalid.
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD168 |
| Target | Canine distemper virus, canine parvovirus and Dirofilaria immitis antigens, and Ehrlichia canis and Leptospira spp. antibodies |
| Specimen | Canine secretions from the eye conjunctiva, nasal cavities or anus (CDV), canine feces (CPV) and canine serum, plasma or whole blood (CHW-EHR-LEPTO) |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (10T) | 10 CDV Ag-CPV Ag-CHW Ag-EHR Ab-LEPTO Ab test cards, 10 vials of CDV assay buffer, 10 vials of CPV assay buffer, 10 vials of CHW-EHR-LEPTO assay buffer, 10 capillary droppers, 30 disposable droppers, 20 specimen collection swabs, 1 instruction for use |
| Pack size | 10 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Canine secretions from the eye conjunctiva, nasal cavities or anus (CDV), canine feces (CPV) and canine serum, plasma or whole blood (CHW-EHR-LEPTO). |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD168 | Canine Distemper Virus-Parvovirus-Dirofilaria immitis Ag, Ehrlichia canis-Leptospira spp. Ab Combo Test | 10 tests / box |
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