IgE Ab

Name: IgE Ab
Brand: Hangzhou Evegen Biotech CO.,Ltd.
Availability: InStock

Cat.No.：

VD139

Name：

Canine Immunoglobulin E Ab Test

Target：

Immunoglobulin E antibodies

Specs：

10 tests/box

Storage:

2-30 °C

Stability:

24 Months

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Product Overview

The Canine Immunoglobulin E Ab Test is a lateral flow immunochromatographic assay for the detection of Immunoglobulin E in canine serum, plasma or whole blood. It can be performed by a dropper method or a pipette method and delivers a visible result in 5 to 10 minutes.

Suitable for:

🐕 Dogs at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & breeding kennels 🔬 Field screening

Key Features

🎯 Instrument-free	Clear visual result in 5–10 minutes — no analyzer required
🧬 Target	Immunoglobulin E antibodies
⚡ Fast result	5–10 minutes
🔬 Highly selective	Target-specific capture and gold-labeled detector antibodies
❄️ Room-temp storage	2–30 °C, no cold chain needed
📦 Flexible packs	10T kit available

Detection Principle

The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. Highly selective antibodies to immunoglobulin E antibodies serve as the capture (T) and gold-labeled detector antibodies. When the treated sample is added to the sample well, the liquid flows laterally across the test strip. If the target is present, a visible T line appears. The C line must always appear after sample application, indicating proper test performance.

Test Procedure

1	Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use.
2	Collect sample — collect serum, plasma or whole blood following standard veterinary procedures.
3	Prepare sample — transfer 1 drop of the collected specimen (using a capillary dropper) into the assay buffer tube and mix thoroughly. Alternatively, add 10 μL of the specimen into the assay buffer tube with a pipette and mix thoroughly. Use the mixed liquid as the treated sample.
4	Apply sample — remove the test card from the foil pouch, place it flat, and transfer 3 drops of the treated sample (using a disposable dropper), or 100 μL (using a pipette), into the sample well.
5	Read result — start the timer and read the result at 5–10 minutes. Do not move the test card during testing.

Result Interpretation

C line	T line	Result	Interpretation
Visible	Visible	Positive (+)	Target detected. Any faintly visible T line is considered positive when the C line is present.
Visible	Not visible	Negative (−)	No target detected.
No line	Any	Invalid	Test failed; repeat with a new test card.

Two manual preparation options are available. Dropper method: add 1 drop of the collected specimen into the assay buffer tube with the capillary dropper, mix thoroughly, then add 3 drops of the treated sample into the sample well with the disposable dropper. Pipette method: add 10 μL of the specimen into the assay buffer tube, mix thoroughly, then transfer 100 μL of the treated sample into the sample well. Read the result within 5 to 10 minutes in both cases.

* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.

Specifications

Cat. No.	VD139
Target	Immunoglobulin E antibodies
Specimen	Canine serum, plasma or whole blood
Format	Lateral flow test card
Reading time	5–10 minutes
Kit contents (10T)	10 IgE Ab test cards, 10 vials of assay buffer, 10 disposable droppers, 10 capillary droppers, 1 instruction for use
Pack size	10 tests / box
Storage	2–30 °C
Shelf life	24 months

Storage & Stability

Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.

FAQ

Q	Which specimens can be tested?
A	Canine serum, plasma or whole blood.
Q	How long until the result?
A	5–10 minutes after adding the sample.
Q	Do I need an instrument?
A	No instrument is required for Method 1 (visual interpretation).
Q	What if the T line is very faint?
A	Any faintly visible T line is considered positive when the C line is present.
Q	Can the test card be reused?
A	No. Each card is for single use only.
Q	What does the C line mean?
A	The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat.