FHW Ag

Cat.No.：

VD128Q

Name：

Feline Heartworm Ag Test

Target：

Dirofilaria immitis antigen

Specs：

10 tests/box

Storage:

2-30 °C

Stability:

24 Months

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Product Overview

The Feline Heartworm Ag Test is a lateral flow immunochromatographic assay designed for the detection of Dirofilaria immitis (heartworm) antigen in feline serum, plasma or whole blood, delivering a fast and visible result within 5–10 minutes.

Suitable for:

🐈 Cats at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & shelters 🔬 Field screening

Key Features

🎯 Dual reading modes	Visual judgment, or analysis with VETSEE G2 Dry Immunoassay Analyzer
🧬 Target	Dirofilaria immitis antigen
⚡ Fast result	5–10 minutes
🔬 Highly selective	Target-specific capture and gold-labeled detector antibodies
❄️ Room-temp storage	2–30 °C, no cold chain needed
📦 Flexible packs	10T kit available

Detection Principle

The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. Highly selective antibodies to Dirofilaria immitis antigen serve as the capture (T) and gold-labeled detector antibodies. When the treated sample is added to the sample well, the liquid flows laterally across the test strip. If the target is present, a visible T line appears, which can also be recognized by the VETSEE G2 analyzer. The C line must always appear after sample application, indicating proper test performance.

Test Procedure

1	Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use.
2	Collect sample — collect serum, plasma or whole blood following standard veterinary procedures.
3	Apply sample — remove the test card from the foil pouch, place it flat, and transfer 1 drop of the sample (using a capillary dropper) into the sample well.
4	Add buffer — transfer 3 drops of the assay buffer (using a disposable dropper) into the sample well.
5	Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing.

* Instrumental method (Standard Test / Quick Test) also available with the VETSEE G2 Dry Immunoassay Analyzer — see the IFU for details.

Result Interpretation

C line	T line	Result	Interpretation
Visible	Visible	Positive (+)	Target detected. Any faintly visible T line is considered positive when the C line is present.
Visible	Not visible	Negative (−)	No target detected.
No line	Any	Invalid	Test failed; repeat with a new test card.

Results can be read visually or semi-quantitatively with the analyzer, which classifies them into five phases: negative, suspicious (grey zone), weak positive, positive and strong positive. A suspicious result is indeterminate and repeat testing is recommended. Any faintly visible T line should be considered positive when the C line is also present, and the test should be repeated if the analyzer result conflicts with the visual judgment.

* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.

Specifications

Cat. No.	VD128Q
Target	Dirofilaria immitis antigen
Specimen	Feline serum, plasma or whole blood
Format	Lateral flow test card
Reading time	5–10 minutes
Kit contents (10T)	10 FHW Ag test cards, 10 vials of assay buffer, 10 disposable droppers, 10 capillary droppers, 1 instruction for use
Pack size	10 tests / box
Storage	2–30 °C
Shelf life	24 months

Storage & Stability

Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.

FAQ

Q	Which specimens can be tested?
A	Feline serum, plasma or whole blood.
Q	How long until the result?
A	5–10 minutes after adding the sample.
Q	Do I need an instrument?
A	No instrument is required for Method 1 (visual interpretation). The VETSEE G2 analyzer is optional for semi-quantitative reading.
Q	What if the T line is very faint?
A	Any faintly visible T line is considered positive when the C line is present.
Q	Can the test card be reused?
A	No. Each card is for single use only.
Q	What does the C line mean?
A	The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat.