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FeLV Ag
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Product Overview
The Feline Leukemia Virus Ag Test is a lateral flow immunochromatographic assay designed for the detection of feline leukemia virus antigen (FeLV Ag) in feline serum, plasma or whole blood, delivering a fast, visible result in 5–10 minutes.
Suitable for:
🐈 Cats at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & shelters 🔬 Field screening
Key Features
| 🎯 Dual reading modes | Visual judgment, or analysis with VETSEE G2 Dry Immunoassay Analyzer |
| 🧬 Target | Feline leukemia virus antigen |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 10T kit available |
Detection Principle
The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. Highly selective antibodies to feline leukemia virus antigen serve as the capture (T) and gold-labeled detector antibodies. When the treated sample is added to the sample well, the liquid flows laterally across the test strip. If the target is present, a visible T line appears, which can also be recognized by the VETSEE G2 analyzer. The C line must always appear after sample application, indicating proper test performance.
Test Procedure
| 1 | Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect sample — collect serum, plasma or whole blood following standard veterinary procedures. |
| 3 | Apply sample — remove the test card from the foil pouch, place it flat, and transfer 1 drop of the sample (using a capillary dropper) into the sample well. |
| 4 | Add buffer — transfer 3 drops of the assay buffer (using a disposable dropper) into the sample well. |
| 5 | Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing. |
* Instrumental method (Standard Test / Quick Test) also available with the VETSEE G2 Dry Immunoassay Analyzer — see the IFU for details.
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed; repeat with a new test card. |
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD022Q |
| Target | Feline leukemia virus antigen |
| Specimen | Feline serum, plasma or whole blood |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (10T) | 10 test cards, 10 vials of assay buffer, 10 droppers, 10 capillary droppers, 1 IFU |
| Pack size | 10 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Feline serum, plasma or whole blood. |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). The VETSEE G2 analyzer is optional for semi-quantitative reading. |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD022Q | Feline Leukemia Virus Ag Test | 10 tests / box |
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