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PCV-Ⅱ Ab Elisa

Catalog Number：

VD510E

Full Name：

Porcine Circovirus type-Ⅱ Antibody Elisa Kit

Detection Target：

Porcine Circovirus Type-Ⅱ Antibody

Specifications：

96T/192T/480T

Storage:

2-8 °C

Stability:

12 Months
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PCV-Ⅱ Ab Elisa
PCV-Ⅱ Ab Elisa

Intended Use

Porcine Circovirus Type-Ⅱ Antibody ELISA Detection Kit is for the determination of specific PCV antibody in  pig’s serum  in vitro. 
The test is used to assess the vaccinated from immunization with porcine circovirus type--Ⅱ vaccine of pig farms.

 

Principle

The test is prepackaged with PCV antigen in micropores of enzyme-labeled lath using indirect ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains anti-PCV specific antibody, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, the enzyme-labeled conjugate is added to bind with specific  antigen-antibody complex on the coated plate. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader.

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