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IBRV Ab Elisa

Catalog Number：

VD105E

Full Name：

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection kit

Detection Target：

Infectious Bovine Rhinotracheitis Virus Antibody

Specifications：

96T/192T/480T

Storage:

2-8 °C

Stability:

12 Months
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IBRV Ab Elisa
IBRV Ab Elisa

Intended Use

The Infectious Bovine Rhinotracheitis Virus Antibody Elisa Detection Kit is used to determine the infectious rhinotracheitis virus antibodies in bovine serum in vitro.

 

Principle

 

The test is prepackaged with Bovine infectious rhinotracheitis antigen in micropores of enzyme-labeled lath using indirect ELISA. In the test, the diluted serum to be tested is added, and after incubation, if the sample contains antibodies against infectious bovine rhinotracheitis virus, it will bind to the antigen on the coated Microplate. After washing to remove the unbound antibody and other components, enzyme-labeled antibodies are added to combine with the antigen-antibody complex in the well to form an antigen-antibody-enzyme-labeled antibody complex. After washing, the unbound enzyme conjugate was removed, the color developing substrate solution was added into the microwell, and the blue signal generated by the enzyme catalysis was proportional to the antibody content in the sample. After the reaction was terminated by adding the stop solution, the absorbance OD value in the reaction well was measured with the wavelength of 450nm using an 96-well microplate reader.

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