NT-proBNP

Cat.No.：

VD155Q

Name：

Feline NT- proBNP Test

Target：

Specs：

10 tests/box

Storage:

2-30 °C

Stability:

24 Months

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Product Overview

The Feline NT-proBNP Test is a lateral flow immunochromatographic assay designed for the quantitative detection of the concentration of N-terminal pro B-type natriuretic peptide (NT-proBNP) in feline serum or plasma, providing a numerical result in about 10 minutes to help evaluate heart failure in cats.

Suitable for:

🐈 Cats at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & shelters 🔬 Field screening

Key Features

🎯 Quantitative result	Analyzer-computed concentration displayed on the VETSEE G2 screen in pmol/L
🧬 Target	N-terminal pro B-type natriuretic peptide
📊 Detection range	50-1500 pmol/L
⚡ Fast result	About 10 minutes
🔬 Highly selective	Target-specific capture and gold-labeled detector antibodies
❄️ Room-temp storage	2–30 °C, no cold chain needed
📦 Flexible packs	10T kit available

Detection Principle

The test is based on a sandwich lateral flow immunochromatographic assay. The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. When the treated sample is applied, the liquid flows laterally across the NC membrane; the target analyte in the sample reacts with the pre-coated monoclonal antibodies and forms a visible T line at the test zone. The optoelectronic system of the VETSEE G2 Dry Immunoassay Analyzer analyzes the signal intensity of the T line against the lot-specific calibration curve (imported by scanning the QR code), and the concentration is displayed directly on the analyzer screen. The C line indicates a valid test result.

Test Procedure

1	Equilibrate — Allow all test components to equilibrate to room temperature (15-25°C) for 10 minutes before use.
2	Turn on the analyzer — Turn on the dry immunoassay analyzer and use its scanner to scan the QR code on the inner label of the box or the label of the bag to import the calibration curve of the lot.
3	Load the test card — Remove the test card from the foil pouch. With the front facing up and the sample well oriented towards the outside of the analyzer's test card slot, insert the card into the slot.
4	Prepare the sample — Use a pipette to add 100 μL of assay buffer into a centrifuge tube, then add 100 μL of the specimen into the same tube and mix thoroughly; use the mixed liquid as the treated sample.
5	Apply the sample — Use a pipette to aspirate the treated sample and add 100 μL of it into the sample well, then push the test card to the farthest end of the test card slot.
6	Run the test — Tap [STANDARD TEST] on the analyzer screen; the result will be displayed in about 10 minutes.

* The result is calculated and displayed by the VETSEE G2 Dry Immunoassay Analyzer (Standard Test); refer to Result Interpretation for the reference range.

Result Interpretation

Concentration	Interpretation
≤100 pmol/L	Normal
100-270 pmol/L	Mild abnormal
>270 pmol/L	Abnormal, possibility of heart failure

The concentration of NT-proBNP is displayed directly on the analyzer screen (detection range 50-1500 pmol/L); interpret the value with the reference range above. When the screen displays "invalid", review the operating procedures and repeat the test with a new card. The suggested reference range was established based on a certain region; it is recommended that each laboratory establishes its own reference range to fit local patients.

* Suggested reference ranges were established based on a certain region; because of varietal and regional differences, each laboratory should establish its own reference range. For in vitro veterinary use only — results should be considered alongside other clinical information.

Specifications

Cat. No.	VD155Q
Target	N-terminal pro B-type natriuretic peptide
Specimen	Feline serum or plasma
Format	Lateral flow test card (quantitative)
Reading time	About 10 minutes (automatic countdown)
Detection range	50-1500 pmol/L
Kit contents (10T)	10 NT-proBNP test cards, 10 vials of assay buffer, 20 pipette tips, 10 centrifuge tubes, 1 label of QR code (calibration curve), 1 instruction for use
Pack size	10 tests / box
Storage	2–30 °C
Shelf life	24 months

Storage & Stability

Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.

FAQ

Q	Which specimens can be tested?
A	Feline serum or plasma.
Q	How long until the result?
A	About 10 minutes after starting the test on the analyzer.
Q	Do I need an instrument?
A	Yes — the VETSEE G2 Dry Immunoassay Analyzer is required; the concentration is calculated and displayed automatically.
Q	How is the concentration reported?
A	The analyzer displays the concentration directly on its screen (detection range 50-1500 pmol/L); compare it with the reference range in Result Interpretation.
Q	Can the test card be reused?
A	No. Each card is for single use only.
Q	What does the C line mean?
A	The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat.