LSH-ANA

Cat.No.：

VD129

Name：

Canine Leishmania-Anaplasma Ab Combo Test

Target：

-Leishmania canis antibody -Anaplasma spp. antibody

Specs：

10 tests/box

Storage:

2-30 °C

Stability:

24 Months

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Product Overview

The Canivet Leishmania-Anaplasma Ab Combo Test is a lateral flow immunochromatographic assay designed for the rapid differential diagnosis of leishmaniasis and anaplasmosis by detecting antibodies specific to Leishmania canis and Anaplasma spp. in canine serum, plasma or whole blood, from a single specimen collection and sample preparation.

Suitable for:

🐕 Dogs at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & breeding kennels 🔬 Field screening

Key Features

🎯 Multi-marker panel	Up to 2 analytes in one test — single specimen, one preparation
🧬 Target	Leishmania canis and Anaplasma spp. antibodies
⚡ Fast result	5–10 minutes
🔬 Highly selective	Target-specific capture and gold-labeled detector antibodies
❄️ Room-temp storage	2–30 °C, no cold chain needed
📦 Flexible packs	10T kit available

Detection Principle

The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 2 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.

Test Procedure

1	Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use.
2	Collect sample — collect serum, plasma or whole blood following standard veterinary procedures.
3	Apply sample — remove the test card from the foil pouch, place it flat, and transfer 1 drop of the sample (using a capillary dropper) into each sample well.
4	Add buffer — transfer 3 drops of the assay buffer (using a disposable dropper) into each sample well.
5	Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing.

Result Interpretation

C line	T line	Result	Interpretation
Visible	Visible	Positive (+)	Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present.
Visible	Not visible	Negative (−)	No target detected.
No line	Any	Invalid	Test failed in the corresponding window; repeat with a new test card.

The two windows are read independently using the same blood specimen. A positive LSH window indicates antibodies against Leishmania canis, while a positive ANA window indicates antibodies against Anaplasma spp. In each window a magenta C line must appear for the result to be valid; a visible T line together with the C line is positive (any faintly visible T line counts as positive), a C line without a T line is negative, and a window without a C line is invalid.

* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.

Specifications

Cat. No.	VD129
Target	Leishmania canis and Anaplasma spp. antibodies
Specimen	Canine serum, plasma or whole blood
Format	Lateral flow test card
Reading time	5–10 minutes
Kit contents (10T)	10 LSH-ANA test cards, 10 vials of assay buffer, 10 disposable droppers, 10 capillary droppers, 1 instruction for use
Pack size	10 tests / box
Storage	2–30 °C
Shelf life	24 months

Storage & Stability

Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.

FAQ

Q	Which specimens can be tested?
A	Canine serum, plasma or whole blood.
Q	How long until the result?
A	5–10 minutes after adding the sample.
Q	Do I need an instrument?
A	No instrument is required for Method 1 (visual interpretation).
Q	What if the T line is very faint?
A	Any faintly visible T line is considered positive when the C line is present.
Q	Can the test card be reused?
A	No. Each card is for single use only.
Q	What does the C line mean?
A	The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat.