FCV-FHV-FCoV-FPV-TOXO Ag

Cat.No.：

VD096

Name：

Feline Calicivirus-Herpesvirus Type1-Coronavirus-Panleukopenia Virus-Toxoplasma Ag Combo Test

Target：

-Feline calicivirus antigen -Feline herpesvirus type-1 antigen -Feline coronavirus antigen -Feline panleukopenia virus antigen -Toxoplasma gondii antigen

Specs：

10 tests/box

Storage:

2-30 °C

Stability:

24 Months

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Product Overview

The Feline Calicivirus-Herpesvirus Type1-Coronavirus-Panleukopenia Virus-Toxoplasma Ag Combo Test is a lateral flow immunochromatographic assay designed for the rapid differential diagnosis of these pathogens by detecting feline calicivirus and feline herpesvirus type-1 antigens in secretions from feline eye conjunctiva or nasal cavities, and feline coronavirus, feline panleukopenia virus and Toxoplasma gondii antigens in feline feces, from a single specimen collection.

Suitable for:

🐈 Cats at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & shelters 🔬 Field screening

Key Features

🎯 Multi-marker panel	Up to 5 analytes in one test — two specimens, independent preparation per window
🧬 Target	Feline calicivirus and feline herpesvirus type-1 antigens; feline coronavirus, feline panleukopenia virus and Toxoplasma gondii antigens
⚡ Fast result	5–10 minutes
🔬 Highly selective	Target-specific capture and gold-labeled detector antibodies
❄️ Room-temp storage	2–30 °C, no cold chain needed
📦 Flexible packs	10T kit available

Detection Principle

The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 5 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.

Test Procedure

1	Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use.
2	Collect sample — collect secretions from the eye conjunctiva or nasal cavities with a swab for the FCV and FHV windows, and fresh feces with a separate swab for the FCoV, FPV and TOXO windows.
3	Prepare sample — insert the secretion swab and the fecal swab into their respective assay buffer tubes, agitate thoroughly, and let them stand for 30–60 seconds. Use the liquid supernatant as sample.
4	Apply sample — remove the test card from the foil pouch, place it flat, and transfer 3 drops of the treated secretion sample into each of the FCV and FHV sample wells and 4 drops of the treated fecal sample into each of the FCoV, FPV and TOXO sample wells.
5	Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing.

Result Interpretation

C line	T line	Result	Interpretation
Visible	Visible	Positive (+)	Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present.
Visible	Not visible	Negative (−)	No target detected.
No line	Any	Invalid	Test failed in the corresponding window; repeat with a new test card.

The five windows are read independently. The FCV and FHV windows detect feline calicivirus and feline herpesvirus type-1 antigens in secretions from feline eye conjunctiva or nasal cavities, while the FCoV, FPV and TOXO windows detect feline coronavirus, feline panleukopenia virus and Toxoplasma gondii antigens in feline feces.

* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.

Specifications

Cat. No.	VD096
Target	Feline calicivirus and feline herpesvirus type-1 antigens; feline coronavirus, feline panleukopenia virus and Toxoplasma gondii antigens
Specimen	Feline secretions from the eye conjunctiva or nasal cavities (FCV-FHV) and feline feces (FCoV-FPV-TOXO)
Format	Lateral flow test card
Reading time	5–10 minutes
Kit contents (10T)	10 FCV-FHV-FCoV-FPV-TOXO test cards, 10 vials of FCV-FHV assay buffer, 10 vials of FCoV-FPV-TOXO assay buffer, 20 disposable droppers, 20 specimen collection swabs, 1 instruction for use
Pack size	10 tests / box
Storage	2–30 °C
Shelf life	24 months

Storage & Stability

Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.

FAQ

Q	Which specimens can be tested?
A	Feline secretions from the eye conjunctiva or nasal cavities (FCV-FHV) and feline feces (FCoV-FPV-TOXO).
Q	How long until the result?
A	5–10 minutes after adding the sample.
Q	Do I need an instrument?
A	No instrument is required for Method 1 (visual interpretation).
Q	What if the T line is very faint?
A	Any faintly visible T line is considered positive when the C line is present.
Q	Can the test card be reused?
A	No. Each card is for single use only.
Q	What does the C line mean?
A	The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat.