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FCoV Ag-FIP Ab
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Product Overview
The Felivet Coronavirus Ag-Felivet Infectious Peritonitis Ab Combo Test is a lateral flow immunochromatographic assay designed for the rapid differential diagnosis of feline coronavirus infection and feline infectious peritonitis by detecting feline coronavirus antigen (FCoV Ag) in feces and antibodies specific to feline infectious peritonitis (FIP Ab) in ascitic fluid, pleural effusion, serum, plasma or whole blood.
Suitable for:
🐈 Cats at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & shelters 🔬 Field screening
Key Features
| 🎯 Multi-marker panel | Up to 2 analytes in one test — single specimen, one preparation |
| 🧬 Target | Feline coronavirus antigen and feline infectious peritonitis antibodies |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 10T kit available |
Detection Principle
The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 2 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.
Test Procedure
| 1 | Equilibrate — Allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect samples — For the FCoV Ag window, collect fresh feline feces with a specimen collection swab from the feline anus or from the ground, with the amount covering about 1/3 to 2/3 of the swab tip. For the FIP Ab window, collect whole blood with an anticoagulant tube, or separate serum or plasma by centrifugation; alternatively, collect ascitic fluid or pleural effusion with a collection tube. |
| 3 | Prepare samples — Insert the fecal swab into the FCoV Ag assay buffer tube and agitate it to release the sample, then use the liquid supernatant as the treated sample. For the FIP Ab window, use serum, plasma or whole blood directly; for ascitic fluid or pleural effusion, add 1 drop of the specimen with a disposable dropper into the FIP Ab assay buffer tube and mix thoroughly to obtain the treated sample. |
| 4 | Apply samples — Remove the test card from the foil pouch and place it flat on a work surface. Transfer 4 drops of the treated fecal sample into the FCoV Ag sample well. For the FIP Ab sample well, add 1 drop of serum, plasma or whole blood with a capillary dropper, then add 3 drops of the FIP Ab assay buffer with a disposable dropper; for ascitic fluid or pleural effusion, transfer 3 drops of the treated (diluted) sample into the FIP Ab sample well. |
| 5 | Read results — Start the timer and read the result at 5–10 minutes. Do not move the test card during testing. If there is no flow within 30 seconds, add 1 drop more of the sample. |
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed in the corresponding window; repeat with a new test card. |
The two windows are read independently with their own specimens: the FCoV Ag window detects feline coronavirus antigen in feline feces, while the FIP Ab window detects antibodies specific to feline infectious peritonitis in ascitic fluid, pleural effusion, serum, plasma or whole blood. In each window a magenta C line must appear for the result to be valid; a visible T line together with the C line indicates a positive result for the corresponding marker (any faintly visible T line should be considered positive), while a C line without a T line is negative and a window in which no line appears in the C zone is invalid.
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD191 |
| Target | Feline coronavirus antigen and feline infectious peritonitis antibodies |
| Specimen | Feline feces (FCoV Ag) and feline ascitic fluid, pleural effusion, serum, plasma or whole blood (FIP Ab) |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (10T) | 10 FCoV Ag-FIP Ab test cards, 10 vials of FCoV Ag assay buffer, 10 vials of FIP Ab assay buffer, 30 disposable droppers, 10 capillary droppers, 10 specimen collection swabs, 1 instruction for use |
| Pack size | 10 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Feline feces (FCoV Ag) and feline ascitic fluid, pleural effusion, serum, plasma or whole blood (FIP Ab). |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD191 | Felivet Coronavirus Ag-Felivet Infectious Peritonitis Ab Combo Test | 10 tests / box |
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