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HbA1c
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Product Overview
The Canine HbA1c Test is a lateral flow immunochromatographic assay designed for the quantitative detection of the concentration of hemoglobin A1c (HbA1c) in canine whole blood, providing an index of long-term glycemic status in dogs.
Suitable for:
🐕 Dogs at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & breeding kennels 🔬 Field screening
Key Features
| 🎯 Quantitative result | Analyzer-computed concentration displayed on the VETSEE G2 screen in 2–20% |
| 🧬 Target | Hemoglobin A1c |
| 📊 Detection range | 2–20% |
| ⚡ Fast result | About 10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 10T kit available |
Detection Principle
The test is based on a sandwich lateral flow immunochromatographic assay. The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. When the treated sample is applied, the liquid flows laterally across the NC membrane; the target analyte in the sample reacts with the pre-coated monoclonal antibodies and forms a visible T line at the test zone. The optoelectronic system of the VETSEE G2 Dry Immunoassay Analyzer analyzes the signal intensity of the T line against the lot-specific calibration curve (imported by scanning the QR code), and the concentration is displayed directly on the analyzer screen. The C line indicates a valid test result.
Test Procedure
| 1 | Equilibrate — Allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Turn on the analyzer — Turn on the dry immunoassay analyzer and use its scanner to scan the QR code on the inner label of the box or the label of the bag to import the calibration curve of this lot. |
| 3 | Load the test card — Remove the test card from the foil pouch, position it front side up with the sample well oriented towards the outside of the analyzer test card slot, and insert the card into the slot. |
| 4 | Apply sample — Collect whole blood with an anticoagulant tube and use it immediately; if not tested immediately, refrigerate at 2–8 °C and use within 24 hours. Use a pipette to place 10 μL of the whole blood sample into the sample well, then use another pipette to place 90 μL of the assay buffer into the sample well, and push the test card to the farthest end of the test card slot. |
| 5 | Run the test — Tap [STANDARD TEST] on the screen; the HbA1c concentration (%) will be displayed automatically at the end of the countdown, about 10 minutes later. |
* The result is calculated and displayed by the VETSEE G2 Dry Immunoassay Analyzer (Standard Test); refer to Result Interpretation for the reference range.
Result Interpretation
| Concentration | Interpretation |
|---|---|
| < 4% | Normal |
| 4–6% | Mild abnormal |
| > 6% | Abnormal; possibility of diabetes |
The HbA1c concentration (%) is displayed directly on the screen of the analyzer (detection range 2–20%); refer to the reference range in practice. When the analyzer displays "invalid", it is recommended to review the operating procedures and perform another test. The suggested reference range was established based on a certain region; because of varietal and regional differences, it is recommended that each laboratory establishes its own reference range to fit local patients.
* Suggested reference ranges were established based on a certain region; because of varietal and regional differences, each laboratory should establish its own reference range. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD195Q |
| Target | Hemoglobin A1c |
| Specimen | Canine whole blood |
| Format | Lateral flow test card (quantitative) |
| Reading time | About 10 minutes (automatic countdown) |
| Detection range | 2–20% |
| Kit contents (10T) | 10 HbA1c test cards, 10 vials of assay buffer, 20 pipette tips, 1 label of QR code (calibration curve), 1 instruction for use |
| Pack size | 10 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Canine whole blood. |
| Q | How long until the result? |
| A | About 10 minutes after starting the test on the analyzer. |
| Q | Do I need an instrument? |
| A | Yes — the VETSEE G2 Dry Immunoassay Analyzer is required; the concentration is calculated and displayed automatically. |
| Q | How is the concentration reported? |
| A | The analyzer displays the concentration directly on its screen (detection range 2–20%); compare it with the reference range in Result Interpretation. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD195Q | Canine HbA1c Test | 10 tests / box |
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