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E.cuniculi IgG IgM
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Product Overview
The Rabbit Encephalitozoon cuniculi IgG/IgM Ab Test is a lateral flow immunochromatographic assay designed for the detection of Encephalitozoon cuniculi antibodies (E.cuniculi IgG and IgM) in rabbit serum, plasma or whole blood. The single testing window carries two test lines so that IgG and/or IgM positivity can be judged visually, or the result can be classified semi-quantitatively into five phases with a dry immunoassay analyzer.
Suitable for:
🐰 Rabbits at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rabbit farms & breeding facilities 🔬 Field screening
Key Features
| 🎯 Dual reading modes | Visual judgment, or analysis with VETSEE G2 Dry Immunoassay Analyzer |
| 🧬 Target | Encephalitozoon cuniculi IgG and IgM antibodies |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 5T kit available |
Detection Principle
The test card features a testing window with an initially invisible T (test) zone and a C (control) zone. Highly selective antibodies to encephalitozoon cuniculi IgG and IgM antibodies serve as the capture (T) and gold-labeled detector antibodies. When the treated sample is added to the sample well, the liquid flows laterally across the test strip. If the target is present, a visible T line appears, which can also be recognized by the VETSEE G2 analyzer. The C line must always appear after sample application, indicating proper test performance.
Test Procedure
| 1 | Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect sample — collect serum, plasma or whole blood following standard veterinary procedures. |
| 3 | Apply sample — remove the test card from the foil pouch, place it flat, and transfer 1 drop of the sample (using a capillary dropper) into the sample well. |
| 4 | Add buffer — transfer 3 drops of the assay buffer (using a disposable dropper) into the sample well. |
| 5 | Read result — start the timer and read at 5–10 minutes. Do not move the test card during testing. |
* Instrumental method (Standard Test / Quick Test) also available with the VETSEE G2 Dry Immunoassay Analyzer — see the IFU for details.
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed; repeat with a new test card. |
The single testing window contains a G (test) line for E.cuniculi IgG, an M (test) line for E.cuniculi IgM and a C (control) line. Read visually 5–10 minutes after sample application: IgG positive when the C and G lines are visible; IgM positive when the C and M lines are visible; IgG and IgM positive when the C, G and M lines are all visible; negative when only the C line is visible; invalid when no line appears in the C zone. With instrumental reading, the result is classified into five phases: negative (low likelihood of E.cuniculi infection), suspicious or grey zone (indeterminate, repeat testing recommended), weak positive (low E.cuniculi IgG and/or IgM antibody titer), positive (middle antibody titer) and strong positive (high antibody titer). The analyzer result is more objective than visual judgment, especially at low antibody titers.
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD198Q |
| Target | Encephalitozoon cuniculi IgG and IgM antibodies |
| Specimen | Rabbit serum, plasma or whole blood |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (5T) | 5 E.cuniculi IgG IgM test cards, 5 vials of assay buffer, 5 disposable droppers, 5 capillary droppers, 1 instruction for use |
| Pack size | 5 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Rabbit serum, plasma or whole blood. |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). The VETSEE G2 analyzer is optional for semi-quantitative reading. |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD198Q | Rabbit Encephalitozoon cuniculi IgG/IgM Ab Test | 5 tests / box |
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