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CPV-CDV-EHR
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Product Overview
The Canivet Parvovirus-Distemper Virus-Ehrlichia Combo Test is a lateral flow immunochromatographic assay designed for the rapid differential diagnosis of these pathogens by detecting canine parvovirus antigen (CPV) in feces, canine distemper virus antigen (CDV) in secretions from the eye conjunctiva, nasal cavities or anus, and antibodies specific to Ehrlichia canis (EHR) in serum, plasma or whole blood.
Suitable for:
🐕 Dogs at risk of infection 🏥 Veterinary clinics & animal hospitals 🐾 Rescue centers & breeding kennels 🔬 Field screening
Key Features
| 🎯 Multi-marker panel | Up to 3 analytes in one test — single specimen, one preparation |
| 🧬 Target | Canine parvovirus and canine distemper virus antigens, and Ehrlichia canis antibodies |
| ⚡ Fast result | 5–10 minutes |
| 🔬 Highly selective | Target-specific capture and gold-labeled detector antibodies |
| ❄️ Room-temp storage | 2–30 °C, no cold chain needed |
| 📦 Flexible packs | 10T kit available |
Detection Principle
The test is based on sandwich method lateral flow immunochromatographic assay. The test card features 3 testing windows, each containing a T (test) zone and a C (control) zone that are initially invisible before the assay. When the treated sample is applied, the liquid flows laterally across the NC membrane of each test strip, interacting with pre-coated target-specific reagents respectively. The presence of target analytes in the sample results in a visible T line, while the appearance of the C line indicates a successful assay.
Test Procedure
| 1 | Equilibrate — allow all components to equilibrate to room temperature (15–25 °C) for 10 minutes before use. |
| 2 | Collect sample — collect fresh feces with a swab for the CPV window and secretions from the eye conjunctiva, nasal cavities or anus with a separate swab for the CDV window; collect whole blood into an anticoagulant tube, or obtain serum or plasma by centrifugation, for the EHR window. |
| 3 | Prepare sample — insert the CPV swab into the CPV assay buffer tube and the CDV swab into the CDV assay buffer tube, agitate to release the sample, and use the liquid supernatant as the treated sample for each window. Use the blood specimen directly for the EHR window. |
| 4 | Apply sample — remove the test card from the foil pouch and place it flat on a work surface. Transfer 4 drops of the treated CPV sample into the CPV sample well and 3 drops of the treated CDV sample into the CDV sample well; for the EHR sample well, add 1 drop of serum, plasma or whole blood with a capillary dropper, then 3 drops of the EHR assay buffer. |
| 5 | Read result — start the timer and read the result at 5–10 minutes. Do not move the test card during testing. For the CPV window, if there is no flow within 30 seconds, add 1 drop more of the sample. |
Result Interpretation
| C line | T line | Result | Interpretation |
|---|---|---|---|
| Visible | Visible | Positive (+) | Target detected in the corresponding testing window. Any faintly visible T line is considered positive when the C line is present. |
| Visible | Not visible | Negative (−) | No target detected. |
| No line | Any | Invalid | Test failed in the corresponding window; repeat with a new test card. |
The three windows are evaluated independently with their own specimens: the CPV window detects canine parvovirus antigen in feces, the CDV window detects canine distemper virus antigen in secretions from the eye conjunctiva, nasal cavities or anus, and the EHR window detects antibodies against Ehrlichia canis in serum, plasma or whole blood. In each window a magenta C line must appear for the result to be valid; a visible T line together with the C line indicates a positive result (any faintly visible T line counts as positive), while a C line without a T line is negative.
* The C and T lines appear as magenta-colored lines. For in vitro veterinary use only — results should be considered alongside other clinical information.
Specifications
| Cat. No. | VD114 |
| Target | Canine parvovirus and canine distemper virus antigens, and Ehrlichia canis antibodies |
| Specimen | Canine fresh feces (CPV), secretions from the canine eye conjunctiva, nasal cavities or anus (CDV) and canine serum, plasma or whole blood (EHR) |
| Format | Lateral flow test card |
| Reading time | 5–10 minutes |
| Kit contents (10T) | 10 CPV-CDV-EHR test cards, 10 vials of CPV assay buffer, 10 vials of CDV assay buffer, 1 bottle of EHR assay buffer, 10 capillary droppers, 20 disposable droppers, 20 specimen collection swabs, 1 instruction for use |
| Pack size | 10 tests / box |
| Storage | 2–30 °C |
| Shelf life | 24 months |
Storage & Stability
Store the test kit at 2–30 °C in a dry place, away from direct sunlight and humidity. Do not freeze. The kit is stable for 24 months from the date of manufacture when stored under recommended conditions.
FAQ
| Q | Which specimens can be tested? |
| A | Canine fresh feces (CPV), secretions from the canine eye conjunctiva, nasal cavities or anus (CDV) and canine serum, plasma or whole blood (EHR). |
| Q | How long until the result? |
| A | 5–10 minutes after adding the sample. |
| Q | Do I need an instrument? |
| A | No instrument is required for Method 1 (visual interpretation). |
| Q | What if the T line is very faint? |
| A | Any faintly visible T line is considered positive when the C line is present. |
| Q | Can the test card be reused? |
| A | No. Each card is for single use only. |
| Q | What does the C line mean? |
| A | The C (control) line must always appear after sample application, indicating proper test performance. No C line = invalid test, repeat. |
Ordering Information
| Cat. No. | Product | Pack size |
|---|---|---|
| VD114 | Canivet Parvovirus-Distemper Virus-Ehrlichia Combo Test | 10 tests / box |
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